Protection of burn-induced skin injuries by the flavonoid kaempferol

摘要

Thermal bum injury induces inflammatory cell infiltrates in the dermis and thickening of the epidermis. Following a bum injury, various mediators, including reactive oxygen species (ROS), are produced in macrophages and neutrophils, exposing all tissues to oxidative injury. The anti-oxidant activities of flavonoids have been widely exploited to scavenge ROS. In this study, we observed that several flavonoids-kaempferol, quercetin, fisetin, and chrysin-inhibit LPS-induced IL-8 promoter activation in RAW 264.7 cells. In contrast with quercetin and fisetin, pretreatment of kaempferol and chrysin did not decrease cell viability. Inflammatory cell infiltrates in the dermis and thickening of the epidermis induced by bum injuries in mice was relieved by kaempferol treatment However, the injury was worsened by fisetin, quercetin, and chrysin. Expression of TNF-a induced by bum injuries was decreased by kaempferol. These findings suggest the potential use of kaempferol as a therapeutic in thermal bum-induced skin injuries.