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Characterization of protein profiles of Aeromonas hydrophila and Aeromonas caviae isolates from fish

机译:鱼类嗜水气单胞菌和豚鼠气单胞菌分离物的蛋白质谱表征

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摘要

In this study, sixteen Aeromonas (A.) hydrophila and A. caviae isolates obtained from fish in Ankara, Turkey were described and compared with two American type strains (A. hydrophila ATCC 7966 and A. caviae ATCC 15468) and characterised by various methods including biochemical, physiological tests and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Whole cell protein (WCP) profiles of these isolates were analyzed using SDS-PAGE. We concluded that the study showed that there wasa similar clonal group origin of the A hydrophila isolates and the reference strain (ATCC 7966). Likewise, there was also a similarity between the A. caviae isolates and the reference strain (ATCC 15468). The results show that SDS-PAGE may be an efficient method to differentiate Aeromonas isolates.
机译:在这项研究中,描述了从土耳其安卡拉鱼中获得的16种嗜水气单胞菌(A. hydrophila)和ca. caviae分离株,并将其与两种美洲型菌株(A. hydrophila ATCC 7966和A. caviae ATCC 15468)进行了比较,并通过多种方法进行了表征。包括生化,生理测试和十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)。使用SDS-PAGE分析了这些分离株的全细胞蛋白(WCP)图谱。我们得出的结论是,该研究表明嗜水A菌株和参考菌株(ATCC 7966)有相似的克隆群起源。同样,在空肠曲霉分离株和参考菌株(ATCC 15468)之间也有相似之处。结果表明,SDS-PAGE可能是区分气单胞菌分离物的有效方法。

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