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Application of glucose-methanol extender to cryopreservation of semen of sex-reversed females rainbow trout results in high post-thaw sperm motility and fertilizing ability

机译:葡萄糖-甲醇增量剂在逆性雌性虹鳟精液冷冻保存中的应用导致高解冻后精子活力和受精能力

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We tested the usefulness of 0.18 M glucose in 9% methanol as an extender for the cryopreservation of semen of sex-reversed rainbow trout females (n = 9). The semen-to-extender ratio of 1:9 and 15 min equilibration were used. Motility and viability parameters as well as the fertilization rate at the eyed stage and hatching were assessed for post-thaw sperm. The fertilization success of cryopreserved semen was about 80% for both 500,000 and 1,000,000:1 sperm-to-egg ratios. The application of the cryopreservation procedure resulted in remarkably high post-thaw sperm motility and viability (55 and 56%, respectively). Our results for the first time show that the post-thaw fertilization ability of rainbow trout sex-reversed female semen can be similar to that of fresh semen at a sperm-to-egg ratio as low as 500,000:1. The sperm motility pattern characterized by low linearity, high amplitude of lateral head displacement and curvilinear velocity seems to be beneficial for the fertilizing ability of thawed sperm. Sperm viability appears to be a reliable indicator of the quality of thawed semen of rainbow trout sex-reversed females. The cryopreservation procedure using glucose-methanol extender after scaling up can be recommended for routine hatchery practice. (C) 2014 Elsevier B.V. All rights reserved.
机译:我们测试了在9%甲醇中添加0.18 M葡萄糖作为增量剂对性反转虹鳟鱼雌性精液(n = 9)进行冷冻保存的有效性。精液与补充液的比例为1:9,平衡时间为15分钟。融化后精子的运动和生存力参数以及受精阶段和孵化率的评估。对于500,000和1,000,000:1的精子/卵子比率,冷冻保存的精液的受精成功率约为80%。冷冻保存程序的应用导致融化后精子的活力和活力显着提高(分别为55%和56%)。我们的结果首次显示,虹鳟鱼性逆转的雌性精液的解冻后受精能力与精液/卵子比低至500,000:1的新鲜精液相似。线性低,头部横向位移幅度大和曲线速度快的精子活动模式似乎对解冻的精子的受精能力有利。精子的活力似乎是虹鳟性反转女性解冻精液质量的可靠指标。对于常规的孵化场实践,建议使用按比例放大后的葡萄糖-甲醇增量剂进行冷冻保存。 (C)2014 Elsevier B.V.保留所有权利。

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    《Aquaculture》 |2014年第null期|共6页
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  • 正文语种 eng
  • 中图分类 水产、渔业;
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  • 入库时间 2022-08-18 10:13:16

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