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Optimizing a sampling strategy for assessing hyporheic invertebrate biodiversity using the Bou-Rouch method: Within-site replication and sample volume

机译:使用Bou-Rouch方法优化用于评估流变型无脊椎动物生物多样性的采样策略:现场复制和样品量

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To determine the optimal combination of sample volume and replication required to assess hyporheic taxa richness and abundance, 10 consecutive one-L Bou-Rouch pump samples were collected from each of 9 wells inserted in a 3 x 3 m grid at two sites on the Rhone River, France. Variable and non-linear relationships between sample volume and numbers of taxa and individuals demonstrate that hyporheic densities cannot simply be expressed per L for comparison among studies that have collected different sample volumes. Our results imply that an optimal sampling design for rapid assessment of hyporheic biodiversity and abundance would need at least five samples of 3-5 L to provide reasonable levels of precision (20%). At both sites, 6-7 replicates of 5 L sufficed to be able to detect a 50% change in mean taxa richness with a power of 0.90 and alpha = 0.10. Although this sampling exercise should be repeated whenever accurate population estimates are needed, it appears that the spatial variance of the hyporheos at a fine scale (<9 m(2)) is great enough to obscure detection of potentially large differences when replication or sample volume is small. [References: 40]
机译:为了确定评估流变类群丰富度和丰度所需的样本量和复制的最佳组合,从插入3×3 m网格的罗纳河两处的9口井中的每口收集10个连续的1升Bou-Rouch泵样品法国河。样本量与分类单元和个体数量之间的可变和非线性关系表明,流变密度不能简单地按L表示,以用于收集不同样本量的研究之间的比较。我们的结果表明,要快速评估流变型生物多样性和丰度的最佳采样设计至少需要5个3-5 L样本才能提供合理水平的精度(20%)。在两个站点上,足够进行6-7次5 L复制,能够检测到平均分类单元丰富度发生50%的变化,功效为0.90,α= 0.10。尽管每当需要准确的种群估计值时都应重复进行这种采样操作,但看起来流变的细微尺度(<9 m(2))的空间差异足以遮盖复制或样本量时潜在的大差异是小。 [参考:40]

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