Phenylboronic acid functionalized gold nanoparticles for highly sensitive detection of Staphylococcus aureus

摘要

Herein, we report a phenylboronic acid functionalized gold nano-particle (GNP)-based colorhnetric assay for rapid detection of Staphylococcus aureus (S. aureus) with high sensitivity. In this approach, GNPs can bind to S. aureus by the reaction of phenylboronic acid with the cis-diol configuration in glycans on the bacterial surface, providing a colorhnetric readout of the binding event. Using this strategy, we have been able to quantify S. aureus at a concentration of 50 cells per mL (three times the standard deviation divided by the slope of the working curve) in aqueous solution.