Investigation on α-Galactosidase Production by Streptomyces griseoloalbus in a Forcefully Aerated Packed-Bed Bioreactor Operating in Solid-State Fermentation Condition

摘要

Solid-state fermentation (SSF) was carried out for the production of extracellular α-galactosidase by Streptomyces griseoloalbus. Soybean flour was the best solid substrate for α-galactosidase production. Packed-bed bioreactor performed well in enhancing the enzyme yield and resulted in a highest yield of 197.2±0.02 U/gds with a forced aeration of 2 vvm, which was approximately twofold the yield obtained in flasks. The α-galactosidase production was growth-associated, and the highest enzyme yield was obtained after 96 h of incubation. It is significant that this is the first report on α-galactosidase production by a filamentous bacterium in SSF using packed-bed bioreactor.