Immobilization of Hemoglobin on the Gold ColloidModified Pretreated Glassy Carbon Electrode for Preparing a Novel Hydrogen Peroxide Biosensor

摘要

A novel hydrogen peroxide (H202) biosensor was developed by immobilizinghemoglobin on the gold colloid modified electrochemical pretreated glassy carbon electrode(PGCE) via the bridging of an ethylenediamine monolayer. This biosensor wascharacterized by UV-vis reflection spectroscopy (UV-vis), electrochemical impendencespectroscopy (EIS) and cyclic voltammetry (CV). The immobilized Hb exhibited excellentelectrocatalytic activity for hydrogen peroxide. The Michaelis–Menten constant (Km) was3.6 mM.