Efficient whole-cell biocatalytic synthesis of N-acetyl-D-neuraminic acid

摘要

N-Acetyl-D-neuraminic acid (Neu5Ac) was efficiently synthesized from lactate and a mixture of N-acetyl-D-glucosamine (GlcNAc) and N-acetyl-D-mannosamine (ManNAc) by whole cells. The biotransformation utilized Escherichia coli cells (Neu5Ac aldolase), Pseudomonas stutzeri cells (lactate oxidase components), GlcNAc/ManNAc and lactate. By this process, 18.32 +/- 0.56 g/liter Neu5Ac were obtained from 65.61 +/- 2.70 g/liter lactate as an initial substrate input. Neu5Ac (98.4 +/- 0.4% purity, 80.87 +/- 0.79% recovery yield) was purified by anionic exchange chromatography. Our results demonstrate that the reported Neu5Ac biosynthetic process can compare favorably with natural product extraction or chemical synthesis processes.