Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum {beta}-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay.

Weldhagen GF

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Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum {beta}-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay.

机译：通过使用实时PCR测定从铜绿假单胞菌的广谱β-内酰胺酶GES-2的快速检测和序列特异性区分。

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The LightCycler was compared to nested PCR for the detection of bla(GES/IBC) genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla(GES/IBC) gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.

机译：将LightCycler与巢式PCR进行了比较，以检测100种铜绿假单胞菌临床分离株中的bla（GES / IBC）基因。实时PCR检测从83个分离株中检测到bla（GES / IBC）基因产物，与巢式PCR和DNA测序相比，其敏感性和特异性分别为94.3和100％。

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《Antimicrobial agents and chemotherapy.》 |2004年第10期|共4页
作者
Weldhagen GF;
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正文语种 eng
中图分类药学;
关键词
Polymerase Chain Reaction; assay; Pseudomonas aeruginosa; Use of; Rapid; 聚合酶链反应; 假单胞菌; 铜绿;

机译：Polymerase Chain Reaction;assay;Pseudomonas aeruginosa;Use of;Rapid;聚合酶链反应;假单胞菌;铜绿;

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1. Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum {beta}-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay. [J] . Weldhagen GF Antimicrobial agents and chemotherapy. . 2004,第10期

机译：通过使用实时PCR测定从铜绿假单胞菌的广谱β-内酰胺酶GES-2的快速检测和序列特异性区分。
2. A nosocomial outbreak of Pseudomonas aeruginosa isolates expressing the extended-spectrum beta-lactamase GES-2 in South Africa. [J] . Poirel L, Weldhagen GF, De Champs C, The Journal of Antimicrobial Chemotherapy . 2002,第3期

机译：铜绿假单胞菌分离株的医院内暴发，在南非表达广谱β-内酰胺酶GES-2。
3. Molecular detection of GES-2 extended spectrum beta-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa [J] . Weldhagen GF, Prinsloo A International journal of antimicrobial agents . 2004,第1期

机译：南非比勒陀利亚生产铜绿假单胞菌的GES-2超广谱β-内酰胺酶的分子检测
4. Carbapenem hydrolyzing beta-lactamases in Pseudomonas aeruginosa [C] . Shizuko Iyobe 7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai . 2000

机译：碳青霉烯水解铜绿假单胞菌中的β-内酰胺酶
5. Rapid and sensitive detection of aflatoxin in animal feeds and food grains using immunomagnetic bead based recovery and real-time immuno quantitative PCR (RT-iqPCR) assay. [D] . Babu, Dinesh. 2010

机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay [O] . Gerhard F. Weldhagen 2004

机译：实时荧光定量PCR快速检测铜绿假单胞菌中广谱β-内酰胺酶GES-2的序列
7. Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay [O] . Weldhagen, Gerhard F. 2004

机译：实时荧光定量PCR技术快速检测铜绿假单胞菌中广谱β-内酰胺酶GES-2的序列特异性

Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum {beta}-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay.

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