首页> 外文期刊>Health Physics: Official Journal of the Health Physics Society >First Insights Into the M2 Inflammatory Response After Adipose-Tissue-Derived Stem Cell Injections in Radiation-Injured Muscles
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First Insights Into the M2 Inflammatory Response After Adipose-Tissue-Derived Stem Cell Injections in Radiation-Injured Muscles

机译:首先洞察M2炎症反应后Adipose-Tissue-Derived干细胞注射在Radiation-Injured肌肉

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摘要

The cutaneous radiation syndrome is the clinical consequence of local high-dose irradiation. It is characterized by extensive inflammation, necrosis, and poor revascularization of the skin, resulting in muscle inflammation and fibrosis. Based on these physiopathological processes, subcutaneous injections of adipose-tissue-derived stem/stromal cells have shown favorable effects on skin-wound healing in a minipig model of cutaneous radiation syndrome, in which muscle fibrosis persisted. Since fibrosis is mainly due to the inflammatory processes that often affect underlying tissues as well, the beneficial effects of intramuscular injections of adipose-tissue-derived stem/stromal cells on tissue recovery were evaluated. The polarization of the inflammatory response of irradiated muscle in a minipig model of cutaneous radiation syndrome was determined after acute local irradiation with 50 Gy gamma rays in a preliminary study (six minipigs). Analysis of the main inflammatory cytokines of the inflammatory response M1 (IL-1-beta and IL-6) and M2 (IL-10 and TGF-beta) by western blotting and in situ hybridization, as well as analysis of CD80/CD206 M1/M2 macrophage-specific markers by immunohistochemistry on minipig muscle samples, was performed 76 d after irradiation. The treatment of irradiated muscles with autologous adipose-tissue-derived stem/stromal cells led to an increase in IL-10 and TGF-beta, being associated with an increase in CD68+/CD206+ cells in this area. This highlights a polarization of M2 in the inflammatory response and indicates that adipose-tissue-derived stem/stromal cells may direct the irradiated tissues' inflammatory response towards a proregenerative outcome.
机译:皮肤的辐射的临床综合征当地大剂量照射的结果。特点是广泛的炎症,坏死了,可怜的血管再生的皮肤,导致肌肉炎症和纤维化。基于这些physiopathological流程,皮下注射adipose-tissue-derived阀杆/基质细胞表现出良好的效果在皮肤伤口愈合的minipig模型皮肤的辐射综合症,肌肉纤维化依然存在。炎症过程的影响基础组织,有益的肌内注射的影响adipose-tissue-derived阀杆/基质细胞组织恢复进行评估。炎症反应的辐照肌肉在一个minipig皮肤的辐射模式综合征急性当地后确定与50 Gy伽马射线辐照初步研究(六minipigs)。炎症的主要炎性细胞因子响应M1 (IL-1-beta和il - 6)和M2 (il - 10和鉴定及免疫印迹和原位杂交以及CD80 / CD206分析M1 / M2 macrophage-specific标记免疫组织化学minipig肌肉样品,辐照后进行76 d。辐照处理与自体肌肉adipose-tissue-derived茎/基质细胞导致il - 10和鉴定及增加与CD68 + / CD206 +细胞的增加在这一领域。平方米的炎症反应和指示adipose-tissue-derived阀杆/基质细胞可能直接辐照组织的炎症吗响应对proregenerative结果。

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