Complexation of Europium(III) with the Zwitterionic Form of Amino Acids Studied with Ultraviolet-Visible and Time-Resolved Laser-Induced Fluorescence Spectroscopy

摘要

The complex formation of europium(III) with the zwitterionic form of amino acids (alanine, phenylalanine, and threonine) has been studied in aqueous solution. Measurements were performed at I velence 0.1 M (NaCl/NaClO_(4)), room temperature, and trace metal concentrations in the range of pH 2 to 8 using ultraviolet-visible (UV-Vis) and time-resolved laser-induced fluorescence spectroscopy (TRLFS). While complexation leads to a significant luminescence enhancement in the emission spectrum of the metal ion, absorption in the UV-Vis spectrum of the amino acid (AA) decreases. As zwitterionic species (AAH), all three ligands form weak complexes with 1:1 stoichiometry and a general formula of EuAAH~(3+) with the metal. The complex stability constants were determined to be log K approx 1 for all complexes, indicating the negligible contribution of the amino acid side chain to the complex formation reaction.