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首页> 外文期刊>Applied radiation and isotopes: including data, instrumentation and methods for use in agriculture, industry and medicine >Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[I-124]iodobenzoate for cell tracking
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Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[I-124]iodobenzoate for cell tracking

机译:用十六烷基-4- [I-124]碘苯甲酸酯跟踪大鼠脂肪性干细胞在大鼠心肌梗死模型中的安全性和有效性

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摘要

This study was aimed to evaluate the effect of I-124-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose derived stem cells (ADSCs). [I-124]HIB showed high labeling efficiency with ADSCs (51.3 +/- 1.3%, 0.3-2.0 Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [I-124]HIB-labeled ADSCs. We demon-strated that I-124-labeling with HIB did not affect the biological properties of ADSCs. (C) 2015 Elsevier Ltd. All rights reserved.
机译:这项研究旨在评估I-124标记的4-碘苯甲酸十六烷基酯(HIB)对与脂肪干细胞(ADSCs)的细胞周期,DNA修复,转录,增殖和分化相关的基因表达的影响。 [I-124] HIB对ADSCs的标记效率很高(51.3 +/- 1.3%,0.3-2.0 Bq /细胞),ADSCs没有形态变化。在基因表达模式的微阵列分析中,未标记的和[I-124] HIB标记的ADSC之间未观察到差异。我们证明了用HIB进行I-124标记不会影响ADSC的生物学特性。 (C)2015 Elsevier Ltd.保留所有权利。

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