A convenient photosynthesis of uniformly [14C]-labelled D-glucose, D-fructose and sucrose, and chemical synthesis of methyl-a-D-glucopyranoside ([U-14C]-glucose)

摘要

This paper describes a convenient procedure for the radiochemical preparation of D-(U-14C]-glucose, d-[U-14C]-fructose and [U-14C]-sucrose with high specific activity by photosynthesis using 'canna indica' leaf, [14C]-carbon dioxide and water in presence of light in a closed system. The [14C]-sugars formed were extracted, separated and then purified by paper chromatography. Further, the pure D-[U-14C]-glucose obtained was converted to methyl-a-D-glucopyranoside ([U-14C]-glucose) by glyco-sidation with methanol using (i) HC1, the conventional Fischer method (ii) heterogeneous organic cation exchange resin (Amberlite IR-120 (H+)) and (iii) heterogeneous inorganic cation exchanged montmor-illonites called metal M+n-monts. The results indicated that the latter in the form of Fe+3-montmorillonite gave a better yield ( 65%) as compared to others (40-56%). The radiochemical purity of the no-carrier added product was more than 98%. The product retained its specific activity as that of the starting material which is in the range of 250-300 mCi/mmole (9.25-11.1 GBq/mmole), suitable for use as a radiotracer in biochemical investigations.