x811, a mutation of Pseudomonas aeruginosa transposable phage D3112 with a pleiotropic effect

摘要

Characteristics of Pseudomonas aeruginosa Transposable Phage D3112 carrying mutation x811 are described. x811 is a recessive mutation with pleiotropic effect. It determines a deteriorated lysis of infected or induced bacteria, a delayed replication and a considerably decreased replication rate. In addition the x811 mutation is expressed as the Kil phenotype, since high-temperature induction of prophage D3112 cts15 x811 does not cause an immediate decrease in the ability of bacteria to form colonies at 42 degC. Restriction analysis of DNA of D3112 cts15 x811 and its segregants has not revealed extended insertions or deletions. The characteristics of segregants of the D3112 cts15 x811 phage agree with the suggestion that the x811 mutation has emerged in a regulatory element that controls both expression of the entire early operon, including the "preearly" function Kil, and the regulation of the repressor synthesis.