Production of Y-chromosome insertions into the left arm of chromosome 2 of Drosophila melanogaster

摘要

An experimental procedure describing production of insertions of Y-chromosome material into autosomes of Drosophila is presented. Irradiated Y:2 translocations served as source material. The insertion selection scheme was based on the emergence of additional progeny classes in the case of independent segregation of the detached fragments of the Y chromosome and autosome .A total of seven insertions of Y-chromosome material into the left arm of chromosome 2, specifically in regions 29F, 34A, and 36B, were obtained. All insertions were lethal in the homozygous state and caused crossing-over suppression in the left arm of chromosome 2. In addition,these mutations induced the formation of loops between the chromocenter and the region of insertion, as well as breaks in one or both homologs, which re frequently observed preparations of polytene chromosomes. The selection scheme suggested can be used to produce insertions in any region of Drosophila melanogaster chromosomes 2 and 3, for which the Y:2 translocations exist.