Inducing synchronous ovarian maturation in the crayfish, Procambarus

摘要

We examined the feasibility of inducing synchronous ovarian maturation in the crayfish, Procambarus clarkii, via eyestalk interventional injection (EI) using a Bletilla striata polysaccharide (BSP) gelatin containing tranexamic acid (TRA), domperidone (DOM) or serotonin (5-HT). In total, 360 females were randomly assigned to seven experimental groups and a control group. The average survival rate (SR%) and average synchronous ovarian maturation rate (SMR%) in survivors in the EI groups, EI-TRA, EI-DOM, EI-5HT were compared with bilateral eyestalk ablation (BEA), unilateral eyestalk ablation (UEA), abdominal injection (AI) of DOM (0.5 mg crayfish(-1)) alone (AI-DOM) or combined with 5-HT (0.5 mg crayfish(-1)) (AI-DOM+5-HT). The experiment covered a prolonged period of 32 days until two ovigerous females were observed in BEA. EI-DOM achieved a SMR (66.67 +/- 9.62%) higher than the control (22.05 +/- 3.06%) (P < 0.05), but lower than BEA (88.89 +/- 11.11%), UEA (63.33 +/- 18.56) and AI-DOM+5-HT (59.26 +/- 10.14%) (P > 0.05). EI-DOM also achieved a higher SR (53.33 +/- 3.85%) than BEA (15.56 +/- 2.22), UEA (24.44 +/- 5.88) (P < 0.05) and AI-DOM+ 5HT (51.11 +/- 4.44) (P > 0.05). However, EI-TRA (SR = 28.89 +/- 4.44, SMR = 37.78 +/- 2.22) and EI-5-HT (SR = 15.56 +/- 4.44, SMR = 22.22 +/- 11.11) failed to induce significantly higher SMR than the control. These findings suggest that EI methods, such as EI-DOM, may have positive characteristics for the development of inexpensive and less labour-intensive techniques for induction of ovarian maturation in decapods