Improvement in wild endangered Persian sturgeon, Acipenser persicus (Borodin, 1897) semen cryopreservation by 2-hydroxypropyl-beta-cyclodextrin (H beta CD)

摘要

In this study, cryopreservation feasibility of Persian sturgeon (Acipenser persicus) and the effect of different doses of 2-hydroxypropyl-beta-cyclodextrin on thawed spermatozoa quality (motility duration and motility percentage) were investigated. For freezing, semen of seven male individuals was pooled in equal volumes and diluted with 4 degrees C [Tris-HCl (100 mM), pH = 8, DMSO 10%] extenders containing 0, 5, 10, 15 mM of H beta CD in a ratio of 1: 1(semen/extenders). Then semen was filled into 0.5-mL straws, and was frozen with vapour of liquid nitrogen at 4-cm above surface of liquid nitrogen. After 3 min, straws were plunged in to liquid nitrogen. Thawing was performed at 40 degrees C water baths for 15 s. Motility duration of the 10 mM H beta CD treated spermatozoa at days 14 (228.98 +/- 16.39) and 56 (199.66 +/- 21.78) were longer than other treatments. In day 56, the motility percentage in treatment with 10 mM was significantly higher (16.14 +/- 2.54) (P 0.05) compared with 5 mM treatment (8.75 +/- 2.47) (P 0.05). Therefore, it is recommended that 10 mM of H beta CD can be used as an additive cryoprotectant for increasing cryopreserved spermatozoa quality in this species.