A reliable method for repetitive bleeding in striped murrel, Channa striata (Bloch).

摘要

Blood is the best choice for minimal invasive tissue sampling in sh, and the method of blood withdrawal is crucial for proper haematological and biochemical analyses. Contamination with enzymes and other constituents from the surrounding tissue is the main source of error with blood sampling (Hile 1982). Therefore, an effective and clean blood sampling method should be used. There are several blood sampling methods for sh in use, including tail ablation, dorsal aorta puncture, cardiac puncture, caudal vein puncture, cardiac severance and caudal vein severance. The most recommended sh blood sampling techniques are cardiac puncture and caudal vein puncture (Blaxhall 1972). Cardiac sampling requires special techniques and experience, as there is the possibility of injury to the heart muscle, which would cause leakage of tissue components into the sample (Gaudet, Racicot & Leray 1975). In caudal vein puncture, the blood vessel is located at the ventral surface of the spine, and experienced technicians should perform the procedure to avoid spinal injury during sampling. Another method of blood sampling rarely practiced is cardinal vein puncture or cuverian duct puncture. This method of blood sampling was carried out in red sea bream, Chrysophrys major (Ikeda, Tsuda, Kurawaka, Fukao & Shirasu 1985) and tilapia, Oreochromis mossambicus (Michael, Srinivas, Sailendri & Muthukkaruppan 1994).