Sperm motility of Rhamdia quelen studied using computer-assisted analysis by open-source software

摘要

Fish sperm quality is often assessed using subjective methods, among which progressive sperm motility and sperm vigour are the ones most frequently observed. However, these measurements have raised doubts regarding their validation because the values obtained vary considerably, and basically depend on the experience of a single observer (Rurangwa, Kime, Ollevier & Nash 2004). Lately, using specific software, fish sperm analyses have been conducted with computer programs such as the computer-assisted sperm analysis (casa) (Ravinder, Nasaruddin, Majumdar & Shivaji 1997; Wilson-Leedy & Ingermann 2007).