Parentage determination of yellow catfish (Pelteobagrus Fulvidraco) based on microsatellite DNA markers

摘要

The application of microsatellite (SSR) markers for parentage determination is a booming science for selective breeding as it can track the ancestry and lineage of fish and maintain pedigree information. This study was initiated to assess the feasibility of SSR markers for parentage determination in yellow catfish (Pelteobagrus fulvidraco) by using simulation and real data analysis. A panel of nine polymorphic SSR markers was chosen from our 454 pyrosequencing transcriptomes to genotype 180 progenies in eighteen yellow catfish full-sib families. These markers were highly polymorphic with a mean of 16.9 alleles per locus and an expected average heterozygosity value of 0.891 in 180 individuals. The allele frequency date-based simulation indicated that the combined exclusion probability values would be over 99 and 99.9 %, respectively, when six and eight SSRs were used. Based on the simulation, all progenies were unambiguously assigned to their putative parental pairs when six SSRs were used, whereas the rate of assignment success for the real data set was only 98.9 %. Moreover, when the number of SSRs was up to nine, all progenies from the eighteen full-sib families were 100 % allocated to their parents. The double-blind test demonstrated that 96 % of the randomly selected progeny can be clustered into their own families. This study demonstrated that the microsatellite-based approach is effective for parentage assignment in yellow catfish and has a significant application in selective breeding in this species.