Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus

摘要

The efficacy of clove oil as an anaesthetic and at producing a physiological response (plasma cortisol and glucose) was evaluated in the kelp grouper, Epinephelus bruneus. To acquire complete anaesthesia in less than 3 min and recovery in <10 min, three doses of clove oil were tested at 18, 22 and 26 pC. Although higher anaesthetic doses resulted in shorter induction times and longer recovery times, and a lower temperature resulted in longer anaesthesia induction and slower recovery, we found the optimal dose and administering temperature of clove oil to be 250-300 mg Lp# at water temperature of 18 pC, 150-200 mg Lp# at water temperature of 22 pC and 50-100 mg Lp# at water temperature of 26 pC respectively. Following the administration of 150 mg Lp# of clove oil at 22 pC, the plasma cortisol level was highest (4.24 pl 1.571 og dLp#) after 12 h and the plasma glucose was highest (92.7 pl 9.61 mg dLp#) after 2 h. These results should be useful to the aquaculture industry, where anaesthesia is necessary for a range of activities.