Chemical composition and osmolality of seminal fluid of Acipenser persicus; their physiological relationship with sperm motility

摘要

Determination of semen quality is necessary to understand the basic biochemical processes occurring during motility of sperm and during fertilization to evaluate the reproductive ability of different fish species and to create an optimal environment for storage of spermatozoa; in this regard less information is available for Acipenseridae compared with Cyprinidae and Salmonidae. The aim of the present study is to determine chemical composition and osmolality of seminal fluid and their relationship with sperm motility in Acipenser persicus. The results obtained show that sodium (Na+), chloride (Cl-) and potassium (K+) were predominant ions in the seminal plasma and the average of osmolality of seminal plasma was 82.56 mOsm kg(-1). The higher chemical contents and osmolality compared with other sturgeon species reveal species-specific characteristics and high secretory activity of spermatic duct in A. persicus. Significant positive correlations were observed between osmolality-Cl-, Na+-osmolality and Na+-Cl- (P<0.05, P<0.001 and P<0.05 respectively). But statistically significant correlation was not observed between seminal plasma parameters and sperm motility. Probably, the Na+ and Cl- are the main electrolytes playing a major role in maintaining the osmolality of the seminal plasma and the viability of the spermatozoa in vivo.