Tests of Mendelian segregation and linkage-group relationships among 31 microsatellite loci in red drum, Sciaenopsocellatus

摘要

Two families (parents and full-sibling progeny) of red drum (Sciaenops ocellatus) were assayed for genotypes at 31 nuclear-encoded microsatellites. Tests of expected Mendelian segregation at 29 of the microsatellites were non-significant following Bonferroni correction; occurrence of null alleles was inferred at the remaining two microsatellites. Nine of the 24 possible linkage groups (range 2-5 microsatellites per linkage group) were identified. Significant differences in recombination rates were found both between sexes and between families. The microsatellite linkage groups generated in this study represent the beginning of a genetic map for red drum, an economically important marine fish in the southeastern region of the United States of America.