Parentage determination in golden mandarin fish (Siniperca scherzeri) based on microsatellite DNA markers

摘要

Retaining pedigree information and genetic diversity in each generation is essential but difficulty in selective breeding of aquaculture species. In this study, simulations and real data analysis were performed to examine the power of microsatellite markers in parentage determination of golden mandarin fish (Siniperca scherzeri). Simulations based on allele frequency data from the population of golden mandarin fish showed that the power of six loci to exclude false parents was higher than 95 % and that of seven loci over 97 %. When marker data from seven loci were combined, the accuracy of assignment to one true parental pair was up to 91.6 % with known parental and filial information. The marker panel tested in mixed families, 89.5 % of progeny was correctly assigned to their parental pairs. The mismatches caused by scoring errors at microsatellites loci were the major reason for the discrepancies between simulations and real data analysis. Taking these results into considerations, it was concluded that microsatellite markers can be used as a tool, alternative to physical tagging, to maintain pedigree information in selection programs of golden mandarin fish.