Putative oligosacharyl transferase from iTrypanosoma brucei/i rhodesiense is conserved in family Trypanosomatidae.

摘要

Current reports on the putative oligosaccharyl transferase (OST) from Trypanosoma brucei rhodesiense indicated that it is evolutionarily conserved within family trypanosomatidae. The study therefore endeavored to test the assertion by attempting to molecularly clone the putative gene in other trypanosome species. An amplicon size of ~2000 bp were amplified in Trypanosoma congolense and Trypanosoma evansi using primers for cloning Trypanosoma brucei rhodesiense putative OST. The PCR bands were akin to reported amplicon size of Trypanosoma brucei rhodesiense putative OST. The data provides evidence that the putative OST gene reported in Trypanosoma brucei rhodesiense was truly evolutionarily conserved in family Trypanosomatidae. The study additionally indicated that this gene is not only conserved between species of the same genus but also between subgenera of genus Trypanosoma.