Effect of calcium disodium EDTA and thiamine on subchronic lead toxicity and oxidative DNA damage in dogs.

摘要

Lead is a heavy dense metal inducing toxicity especially in young animals and children. We describe effects of calcium disodium and thiamine on sub-chronic lead acetate drench in dogs assessed by serum malondialdehyde, gluthathione and comet assay forDNA damage. Groups of 5 pound dogs each were drenched 15mg/kg lead acetate daily for 60 days with or without calcium disodium EDTA (ca-EDTA) 110mg/Kg subcutaneously (SC) twice daily for 3 days, thiamine 25mg/Kg SC twice daily for 7 days and 110mg/kg ca-EDTA 110mg/Kg SC twice daily for 3 days with 25mg/kg thiamine SC twice daily for 7 days. Jugular blood was taken 15 days after the end of the 60 day treatments for serum malondialdehyde, glutathione and comet assay. We found lower glutathione in all dogsgiven lead but the decrease was lower (P < 0.05) in dogs treated with calcium disodium or thiamine. We observed a similar trend with malondialdehyde which increased markedly in lead controls and less (P < 0.05) in dogs given EDTA or thiamine. Our results show a showed a high repair rate of DNA after treatment with thiamine and Ca-EDTA compared with treatments. We find glutathione and malondialdehyde and comet assay can be useful biomarker for lead exposure. We find that thiamine and Ca-EDTA protected dogs most against lead exposure compared with other treatments.