Corpora lutea were confirmed by trans-rectal ultrasound in 11 multiparous and 4 primarous mixed breed dairy cows and then injected 1052mcg cloprostenol sodium (PF2). After 72-96 hours, all cows were inseminated then injected 8 mcg gonadotropin releasing Hormone (GnRH) after confirming preovulatory follicle by TRUS. After 11 days all cows were re-injected with GnRH. After 40-50 days of insemination conception was confirmed by trans-rectal ultrasound. Five multiparous cows became pregnant in the left (3) and right (2) uterine horns whereas all primarous cows in the right horn.
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