Single cell protein production by soil Raoutella ornithinolyticaincubated on waste potato, paper and corn cob products.

摘要

We describe single cell protein (SCP) productionby Raoutella ornithinolytica bacteria isolated from soil and cultured on potato, paperand corncob starch waste residue. We identified 3 strains (A1-3) of R. ornithinolyticaby colony characteristics, microscopy and Kliger, urease, citrate, indole, catalalase,APUI 20, Vitek2 and motility tests finding that A1 was most productive. Byinoculating 0.5ml/100ml R. ornithinolytica A1 on 10g/100 ml potato waste mediafor 72 hours at 50C and pH 8.5 we obtained a SCP with 24.4% protein, carbohydrate24,6%, fat 17,9%, ash 21.8% with humidity of 88.6%, DNA 1.235% and RNA 1.012%.The SCP contained very high levels of essential amino acids. Essential and nonessentialamino acids constituted 93.9 mg/100g. Copper constituted 0.002,phosphorus 3.90, zinc 0.00056, calcium 0.085, potassium 0.132 and sodium 3.86%.