Histopathology and F1L gene sequence analysis of contagious ecthyma virus in sheep and goats.

摘要

Contagious ecthyma, also known as Orf, is a common viral skin disease of sheep and goats caused by Parapoxvirus. We describe pathology and Orf virus F1L gene sequence by PCR from sheep and goats. Tissue samples revealed epidermal hyperplasia with prominent rete ridges, hydropic degeneration of the necrotic keratinocytes, eosinophilic intracytoplasmic inclusion bodies in vacuolated cells and subcorneal pustules. By PCR we found ~50% positive to the virus of which 10 were sequenced for Orf-059-Shiraz strain recorded in GeneBank for phylogenetic analysis and suggested that this gene is highly conserved.