Effects of BME amino acids, alanine, vitamins E and B12 in diluents on goat sperm before and after cryopreservation.

摘要

Semen from 5 adult 2-4 year old Markhoz goats trained to ejaculate and weighing 55-60 kg was cryopreserved in solutions of alanine with or without BME amino acid, vitamins E and/or B12. Controls were only diluent. Sperm quality before freezing, thawing at 5C and 37C were compared for kinetics, motility, viability, acrosome and membrane integrity. At 5C, BME amino acid improved sperm quality ~6-18% compared with controls or other treatments (p <0.05). Other treatments reduced quality ~2-35% but mostly with alanine + vitamin E by ~31-35%. At 37C we found that BME with or without alanine or vitamins did not improve most sperm characteristics. However, BME improved viability ~31.5% whereas other treatments reduced (p≤0.05) it. At 37C, alanine with orwithout BME and vitamins E and B12 reduced (p≤0.05) acrosomes 8-10% and membrane integrity ~10.2% but BME alone boosted acrosomes 6% (P < 0.05). Other parameters were reduced 17-41%. Possibly changes in osmosis caused by BME amino acids may affect watercontent in sperm and reduce ice crystallization and cold shock on membranes. We find that BME amino acids with or without alanine + vitamin B12 may improve some characteristics of thawed goat semen.