Monoclonal and polyclonal antibodies are used for indirect flow cytometric, ELISA and cytotoxicity assays. We report a polyclonal antibody against mouse IgG3 in rabbit. IgG3 was purified by sepharose beads conjugated with protein for affinity chromatography and its purity confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). We then injected New Zealand white rabbit with IgG3 intramuscularly and intradermally. We further purified antibody in rabbit serum with ammonium sulfate precipitation and ion-exchange chromatography and SDS-PAGE. This antibody was conjugated with horse radish peroxidase for titer of rabbit anti-mouse IgG3 and HRP of 64000 determined by ELISA. Purity reached ~95% with optimum dilution -1:10000.
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