Expresseion of porcine adipocyte transcripts during differentiationin vitro and in vivo

摘要

Transcript concentarations for the transcription factors,CCAAT enhancer binding protein #beta# and #alpha#(C/EBP#beta and C/EBP#alpha#),plus the adipocyte-characteristic proteins,fatty acid synthase (FAS),glucose transporter 4 (Glut 4),hormone-sensitive lipase (HSL),insulin receptor (InsR),lipoprotein lipase (LPL),insulin receptor (InsR),lipoprotein lipase (LPL),and leptin were measured during differentiation of porcine stromal-vascular (S/V) cels in vitro.These same transcripts,excluding FAS and InsR,were measured in porcine adipose tissue from Birth to 7 weeks of age.In S/V cells,C/EBP#beta# and InsR ,were measured in porcine adipose tissue from birth to 7 weeks of age.In S/V cells,C/EBP#beta# and InsR were continuously elevated.At day 0,C/EBP#alpha# was approx=20% of the day 9 value.The LPL increased gradually from day 0 to 9,whereas most other transcripts had a lag period of several days.In tissue,C/EBP#beta# was substantial at birth and increased gradually.The C/EBP#alpha# was relatively low at birth and increased at day 17.The LPL and leptin increased continuously.The Glut 4 was low at birth and increased at day 28.The HSL was relatively low at birth,increased at day 10,and plateaued at day 28.Transcripts in porcine S/V cells develop somewhat differently from adipocyte differentiation models established in clonal cells,but the porcine cells represent a model that should be more applicable to pigs.