Clkoning,tissue distribution and nutritional regulation of a #DELTA#6-desaturase-like enzyme in rainbow trout

摘要

This report describes the cloning, nutritional regulation and tissue distribution of a desaturase-like enzyme in rainbow trout (Oncorhynchus mykiss). The open reading frame of the trout desaturase-like cDNA encodes a 454-amino acid peptide that contains two membrane-spanning domains, three histidine-rich regions and a cytochrome bs domain, which all align perfectly with the same domains located in other recently identified vertebrate #DELTA#5-and #DELTA#6-desaturases. Nutritional regulation of trout desaturase-like gene expression, as well as the tissue expression profile, are also similar to those observed in other vertebrate #DELTA#5- and #DELTA#6-desaturases. Finally, the sequence alignments between the predicted protein sequence of rainbow trout desaturase-like and other #DELTA#6- and #DELTA#5-desaturases revealed a high percentage identity with #DELTA#6-desaturases (64-66% identity with vertebrate #DELTA#6-desaturases). These results demonstrate for the first time the presence and nutritional modulation of a #DELTA#6-desaturase-like cDNA in rainbow trout.