首页> 外文期刊>Biochimica et biophysica acta: international journal of biochemistry and biophysics >Retinoic acid repressed the expression of c-fos and c-jun and induced apoptosis in regenerating rat liver after partial hepatectomy.
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Retinoic acid repressed the expression of c-fos and c-jun and induced apoptosis in regenerating rat liver after partial hepatectomy.

机译:维甲酸抑制部分肝切除术后再生大鼠肝脏中c-fos和c-jun的表达并诱导细胞凋亡。

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摘要

Retinoic acid (RA), which was injected within 4 h after partial hepatectomy (PH), inhibited DNA synthesis in regenerating liver. The inhibition was accompanied by apoptosis, evidenced by in situ end labeling and gel electrophoresis of DNA fragmentation. Characteristic DNA fragmentation was obvious at 4 h and reached a maximum at 8 h after injection. Northern blot analysis revealed that RA repressed the expression of c-fos and c-jun at 15 and 30 min with the up-regulation of retinoic acid receptor gamma (RARgamma) and RARbeta at 2 h after PH. The transglutaminase II mRNA level and activity were increased by RA injection at 4 h and 8 h after PH, respectively. The mRNA levels of thymidylate synthase and thymidine kinase, which are rate determining enzymes of DNA synthesis, decreased in RA injected rats. No change was seen in the expression of p53 and p21WAF1/CIP1 which have been suggested to participate in the apoptosis process. These results suggest that RA exerts the antiproliferative activity only on the early stage of liver regeneration accompanied by the repression of c-fos and c-jun expression and induction of apoptosis.
机译:在部分肝切除术(PH)后4小时内注射的维甲酸(RA)抑制了再生肝脏中DNA的合成。抑制伴随有凋亡,这通过原位末端标记和DNA片段的凝胶电泳证明。特征DNA片段在4 h时很明显,并在注射后8 h达到最大。 Northern印迹分析显示,RA在PH后2小时抑制了15-和30分钟时c-fos和c-jun的表达,同时维甲酸受体γ(RARgamma)和RARbeta的表达上调。 PH注射后4 h和8 h分别通过RA注射增加了转谷氨酰胺酶II的mRNA水平和活性。胸腺嘧啶合酶和胸苷激酶的mRNA水平是DNA合成的决定酶,在RA注射的大鼠中降低。 p53和p21WAF1 / CIP1的表达未见变化,提示它们参与了细胞凋亡过程。这些结果表明RA仅在肝再生的早期伴随着c-fos和c-jun表达的抑制以及细胞凋亡的诱导而发挥抗增殖活性。

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