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Therapeutic application of RNA interference against foot-and-mouth disease virus in vitro and in vivo.

机译:RNA干扰在体外和体内对口蹄疫病毒的治疗应用。

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Foot-and-mouth disease (FMD) is an economically important animal disease because of the speed of its transmission. Routine vaccination may not be effective for early protection in an outbreak situation. Small interfering RNA (siRNA) can be used in a rapid and effective antiviral approach. However, siRNA has limitations when used in disease prevention, such as a short duration of action. In this study, we have demonstrated that treatment with siRNA after FMD virus (FMDV) infection has an antiviral effect and could be effective in control of FMDV. We applied adenoviruses expressing siRNA both before and after FMDV infection in vitro and in vivo. Treatment after FMDV infection gave effective viral inhibition, but a combination of treatment before and after FMDV infection gave the best results in IBRS-2 cells. We obtained high survival rates in suckling mice by the use of therapeutic injections following challenge. The results of this study suggest that treatment with siRNA could enhance antiviral effects and may be helpful in the control of FMDV in an outbreak.
机译:口蹄疫(FMD)由于其传播速度快,是一种经济上重要的动物疾病。常规疫苗接种可能无法在疫情暴发时对早期保护有效。小干扰RNA(siRNA)可用于快速有效的抗病毒方法。但是,当用于疾病预防时,siRNA具有局限性,例如作用时间短。在这项研究中,我们已经证明,FMD病毒(FMDV)感染后用siRNA进行治疗具有抗病毒作用,并且可以有效控制FMDV。我们在体外和体内在FMDV感染前后都应用了表达siRNA的腺病毒。 FMDV感染后的治疗可有效抑制病毒,但FMDV感染前后的联合治疗在IBRS-2细胞中效果最佳。通过挑战后使用治疗性注射,我们在乳鼠中获得了较高的存活率。这项研究的结果表明,用siRNA进行治疗可以增强抗病毒作用,并且可能有助于控制暴发中的FMDV。

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