Establishment of the Saliva Volatomic Profile as an Exploratory and Non-invasive Strategy to Find Potential Breast Cancer Biomarkers

摘要

Breast cancer (BC) is a major health problem worldwide. It is the most common malignancy in women and the second cause of cancer-related mortality. BC late diagnosis is a major cause of BC morbidity and improved diagnosis tools are needed. Here we present a sensitive assay aiming to identify volatile organic metabolites (VOMs) as biomarkers of BC development using saliva samples. Saliva is a promising source of VOMs that can be used as potential cancer biomarkers. The extraction of saliva VOMs was performed using dynamic headspace solid phase microextraction (dHS-SPME). Several parameters that influence the extraction were tested. These included the nature of the adsorptive phase, sampling temperature, extraction time, volume, pH, salt concentration and agitation.