The Class III Biological Safety Cabinet (BSC) provides maximum personnel protection when it is adequately field certified and properly used. One of the major criteria for this protection is proper performance of the Class III BSC integrity (tracer gas) test as described in the National Institutes of Health Laboratory Safety Monograph (NCI, 1979). This article deals with the origins, appropriateness, and overall understanding of this essential Class III BSC factory acceptance and field certification test.
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