In vitro callus induction and plant regeneration in nodal explants of Tinospora cordifolia (Willd.) Miers. ex Hook. f. & Thoms.

摘要

An in vitro plant regeneration protocol was developed through callus induction using nodal explants of Tinospora cordifolia. Nodal explants produced callus at all the four concentrations (0.5, 1.0, 2.0 and 4.0 mg/l) of IAA, NAA and 2,4-D on both Murashige and Skoog (MS) and Woody Plant Medium (WPM). The per cent response was maximum (80%) at 4 mg/l 2,4-D on WPM. WPM was better as a basal medium for callus induction and plant regeneration. No callusing was observed in control cultures. The callus was cultured on different concentrations of BAP and KIN for organogenesis. Maximum shoot regeneration (70%) was achieved with 2 mg/l BAP on WPM. In vitro rooting was achieved by transferring the regenerated shoots to half strength WPM supplemented with 0.5 mg/l IBA. Acclimatized plantlets were grown in polyhouse with 70% survival rate.