COMPARISON OF TIME-RESOLVED FLUOROIMMUNOASSAY FOR DETERMINING HEXOESTROL RESIDUES IN CHICKEN MUSCLE TISSUES BASED ON POLYCLONAL ANTIBODIES WITH LIQUID CHROMATOGRAPHY AND TANDEM MASS SPECTROMETRY

摘要

A time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of hexoestrol (HES) residues in animal tissues.The limit of detection (LOD) was determined to be 0.02 ng g-1 and the limit of quantification (LOQ) was less than 0.12 ng g-1.The results obtained by the TR-FIA and ELISA showed a good correlation.The established TR-FIA was validated for the determination of market chicken muscle tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS).This proposed technique could be applied to routine residue analysis.