Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One-Use’Sensing in-a-Drop’Applications

摘要

Stabilization of biomolecules on matrices is critically important.Here we constructed composite nanofibers with magnetic features by electro-spinning of magnetic nanoparticles (MNP) in an appropriate polymer matrix on a collector surface for multiple uses.’Poly(vinylalcohol)-polyethyleneimine/Fe3O4 magnetic nanoparticles’(PVA-PEI/MNP) composite nanofiber was used on the screen printed electrodes (SPE) to serve as a platform for the glucose oxidase (GOx) biofilm as the model biomolecule.To show the potential application of this material as a biosensor component,the resulted biofilm,called’PVAPEI/MNP/GOx’,was fixed on SPE via a neodymium magnet for the electrochemical detection of glucose at-0.7 V where oxygen consumption due to enzymatic reaction was measured.The main advantage of the obtained magnetic biomembrane is that it allows analysis with a single drop.After step-by-step surface modifications,analytical characterization was performed using various techniques,such as voltammetry,electrochemical impedance spectroscopy,Fourier-transform infrared spectroscopy and X-Ray photoelectron spectroscopy as well as microscopic techniques.The linear range of the PVA-PEI/MNP/GOx surface was found as 0.0125 to 0.5 mM with a limit of detection of 11.5 μM for glucose as analyte.The application of PVA-PEI/MNP/GOx for glucose detection in synthetic samples was carried out.Data proved the potential use of the’PVA-PEI/MNP’surface as a biomolecule immobilization platform for disposable biosensor applications.