Glycogen synthase kinase 3bate induces cell cycle arrest in a cyclin Dl- dependent manner in human lung adenocarcinoma cell line A549

摘要

The effect of glycogen synthase kinase 3bate (GSK3bate) has been repeatedly implicated in cell proliferation, but studies on the effect of GSK3P in different cell lines with different stimuli have drawn different conclusions. To investigate the directeffect of GSK3P on cell growth in human lung adenocarcinoma cell line A549, we changed its activity by transient transfection with two kinds of GSK3bate mutant plasmids, constitutively active form S9A-GSK3P and dominant negative form KM-GSK3bate. Twenty-four hours later, cell counting, flow cytometry and Western blot detection were made respectively. The results showed that enhancing GSK3bate activity caused a decrease in cell number, as well as a higher percentage of cells at G_1 phase. Further, the expression of cyclin D_l was down-regulated by GSK3p. Taken together, our observations suggest that GSK3P may induce G_1 cell cycle arrest in a cyclin D_l-dependent fashion and therefore possibly plays a growth-inhibitory role in A549 cells.