Artificial Inoculum and Inoculation Techniques Commonly Used in the Investigation of Fusarium Head Blight in Cereals

摘要

Fusarium head blight (FHB) is a disease of small-grain cereals that has a proven negative impact on crop yield, quality and food safety. In this regard, it is one of the most studied diseases of small-grain cereals worldwide. This paper reports the commonly used artificial sources of Inoculum and inoculation techniques employed in the study of FHB epidemiology. Spore suspensions and grain spawn are the most popular forms of artificial Inoculums. Spray inoculation technique Is more commonly used thanthe point Inoculation technique for delivering the inocula to the target sites. Spray inoculation has an advantage over point inoculation in that it can be used to detect both Type I and I! resistances to FHB, point inoculation can only detect Type II resistance to FHB. Grain spawn is the commonest soil-surface inoculum used In the study of FHB. Its advantage lies in the fact that it is capable of propagating Inoculum over a long period of time compared to other methods. It can al.so detect the five types of resistances reported (Types I, II. Ill, IV and V). In order to gain further information regarding the epidemiology of FHB, researchers need to explore other potential sources of artificial inoculum.