Molecular Imaging Contrast Properties of Fe3O4-Au Hybrid Nanoparticles for Dual-Mode MR/CT Imaging Applications

摘要

The development of noninvasive testing techniques in medi- cine by combining dual-mode Magnetic Resonance (MR)/ Computed Tomography (CT) imaging techniques will help overcome the limitations of the separated methods, leading to more accurate diagnoses. This has implications in clinical research, but currently remains a major challenge in terms of the need for a corresponding multimodal signal contrast agent. In this study, we use small size, monodispersed Fe3O4 magnetic nanoparticles as the seeds to synthesize magneto-plasmonic Fe3O4@Au hybrid nanoparticles (HNPs) with multiple functions with core-shell structures. The influence of the ratios of HAuCl4 and oleyamine (OLA) precursors on the shape and size of the nanoparticles (NPs) was investigated. The HAuCl4/OLA ratio of 0.2/1 is the optimal condition. The obtained NPs has an average size of 21.2 nm with a uniform spherical shape, high mono- dispersity. After the surface functionalization with poly(acrylic acid) (PAA), the resulting Fe3O4@Au@PAA HNPs showed excellent dispersion in water, good biocompatibility, non- toxicity against Hep-G2 cancer cell line and Vero healthy cell line at high test concentration.