Real-Time Detection of Fibrinogen via Imprinted Recognition Sites

摘要

In this study, the fibrinogen imprinted surface plasmon resonance (SPR) biosensor was developed for the monitoring of fibrinogen levels in patient serum samples using a micro- contact imprinting method. The fibrinogen imprinted and non- imprinted polymeric films on the SPR chip surface were prepared by the microcontact imprinting approach and characterized by ellipsometer, contact angle measurements, and Fourier transform infrared spectroscopy. The limit of detection and quantification values of the fibrinogen imprinted SPR biosensors in the linear range of 0.05-9.0 mg/mL were 0.00066 and 0.00219 mg/mL, respectively. In all kinetic ana- lyzes, the response time was 11 min for equilibration, adsorp- tion, and desorption cycles. The selectivity studies of SPR biosensors were performed in the presence of fibrinogen, myoglobin, hemoglobin, and immunoglobulin G in a compet- itive manner. For the validation studies of the fibrinogen imprinted SPR biosensor, the fibrinogen level of diluted patient serum samples was determined by enzyme-linked immuno- sorbent assay (ELISA). The experimental results show that the serum samples of patients in high and low-risk groups were identified with the recovery of about % 97-99 according to both SPR biosensor and ELISA analysis results.