Identification of new T1BL.1RS translocation lines derived from wheat (Triticum aestivum L. cultivar 'Xiaoyan No. 6') and rye hybridization

摘要

Two new T1BL.1RS translocation lines, 48112 and 89121, derived from cross between common wheat (Triticum aestivum L.) cultivar "Xiaoyan No. 6" and rye (Secale cereale L.) cultivar "German White", were developed and identified by using of molecular markers and cytogenetical methods, GISH and FISH. PCR results of primers NOR-R1 specific for rye and Glu-B3 for IBS detected the presence of IRS chromatin and absence of IBS, and primer for gene IBxl4 in 1BL indicated the existence of chromosome arm 1BL inthe two lines. GISH and FISH methods confirmed the replacement of chromosome arm IBS with IRS. Further stripe rust resistant test and quality analysis demonstrated that the new 1BL.IRS translocation lines were higher resistant to mixed races of P. striiformis Westend and observed considerable better quality than other popularized T1BL.IRS cultivars in China. The two lines have been used in wheat breeding for high-yield potential and rust resistance.