A Biosensor for Detection of miR-106 a by Using Duplex-Specific Nuclease,Assisted Target,Magnetic Nanoparticles,Gold Nanoparticles and Enzymatic Signal Amplification

摘要

The second main reason of Cancer mortality all around the world is Gastric cancer.One of the circulatory oncogenic microRNAs which has an overexpression in diverse malignancies,specifically in Gastric cancer,is MicroRNA-106a.In this paper novel supersensitive electrochemical biosensor for the microRNA-106a detection was studied using a duplex-specific nuclease-assisted target recycling incorporated with enzymatic signal amplification,and Fe3O4 nanoparticles.Specific and selective microRNA-106a detection with a low limit of detection(0.8 fM)over a vast concentration range from 1 fM to 5 μM has been achieved using the biosensor.Meantime,the biosensor has dedicated high discrimination of analogue microRNA because of intrinsic selectivity of the term hairpin capture probe.Analyzing microRNA-106a in human serum affirmed the high possibility of the mentioned method for clinical detection of microRNA-106a and its optimistic potential in biomedical investigation.