A Naphthalimides-Based Probe for Sequential Detection of Cu~(2+) and PPi and Its Application in Cells Imaging

摘要

A novel naphthalimides-based fluorescent probe (NPHD) selectively detected Cu~(2+) and PPi through "strong yellow-weak green-strong yellow" photonic response in a sequential manner. The formation of NPHD-Cu~(2+) resulted in obvious fluorescence decreased and wavelength shift from 595 nm to 525 nm by adding Cu~(2+) to NPHD solution. The mechanism could be attributed to d→π* electron transfer (ET) from Cu~(2+) to NPHD. Furthermore, the NPHD-Cu~(2+) complex exhibited high selectivity toward PPi through via emission recovery of chemo-sensor NPHD. The detection limits of NPHD to Cu~(2+) and NPHD-Cu~(2+) to PPi were estimated to be 5.72×10~(8)M and 2.39×10~(8)M. In addition, NPHD could be employed to detect exogenous Cu~(2+) and PPi in Hep G2 cells.