An Enzymatic Glucose Biosensor Based on a Pencil Graphite Electrode Modified with Naphthalenedimide/3,4-Ethylenedioxythiophene Conjugated Polymer and Enriched with Au Nanoparticles

摘要

N,N'-bis(2-hexyl)-2,6-(3,4 ethylenedioxythiophene)-1,4,5,8-naphthalenimide(ENDI)monomer was electropolymerized on pencil graphite electrode(PGE)and used as a biosensor for glucose immobilization for the first time.After electropolymerization of ENDI monomer on PGE(AuNPs/PENDI/PGE),the intensity of the current signal was improved by using gold nanoparticles(AuNPs).The immobilization of the glucose oxidase(GOx)enzyme was carried out by using the cross-linking reagent glutaraldehyde(GA)due to the strong π-π* interaction of the poly-N,N'-bis(2-hexyl)-2,6-(3,4 ethylenedioxythiophene)-1,4,5,8-naphthalenimide(PENDI)polymer.GOx/AuNPs/PENDI/PGE designed biosensor,was characterized by a wide linear glucose determination range between 0.0009-0.33 mM with a limit of detection of 0.0407 mM and a sensitivity of 0.172 μA/mMcm~2.Herein we have shown that GOx/AuNPs/PENDI/PGE biosensor effectively provides the enzyme immobilization and very effective for glucose determination in commercial samples.