Preparation, biodistribution and dosimetry study of Tc-99m labeled N-doped graphene quantum dot nanoparticles as a multimodular radiolabeling agent

摘要

A new Tc-99m N-doped graphene quantum dot Tc-99m-(N-GQD) moiety was developed as a radiolabeling agent for molecules lacking chelation or water solubility of ligands simultaneously. (N-GQDs) were synthesized and characterized with high purity (size range = 5-10 nm). After radiolabeling, ITLC demonstrated that the Tc-99m-(N-GQDs) were prepared with high radiochemical purity (RCP >= 95%). Filtration of Tc-99m-(N-GQDs) with a 0.1 mu m filter proved that 77.38% of the particles were less than 0.1 mu m. Tc-99m-(N-GQDs) were injected into healthy Wistar rats for biodistribution studies. Results showed that Tc-99m-(N-GQDs) were eliminated by the kidneys, and accumulated in the spleen due to the particle size of Tc-99m-(N-GQDs). The ovaries and uterus showed a greater concentration of Tc-99m-(N-GQDs) within 2 h after injection compared with other organs. Dosimetry studies were done to find out the effective radiation dose of each organ, and introduced the kidneys as critical organs. This research has demonstrated the profound feasible production of Tc-99m-(N-GQDs) for possible radiolabeling of molecules with chelation limitations and poor water solubility.