HPLC Determination of Formaldehyde in Flour Samples using 2,4,6-Trichlorophenyl Hydrazine as Derivatization Reagent

摘要

A new simple and sensitive high-performance liquid chromatography (HPLC) method for the determination of formaldehyde in flour samples has been developed. Formaldehyde was quantified after derivatization with a readily available reagent, 2,4,6-trichlorophenyl hydrazine (TCPH) under basic conditions. The formaldehyde-TCPH derivative was eluted with chromatographic mobile phase of 70:30 (v/v) acetonitrile:water at a flow rate of 1.0 mL min-1; wavelength, 222 nm; injection volume, 50 uL, using a CI8 ODS Hypersil column (250 mm x 4.5 mm, 5 urn). The calibration curve was linear over the range of 0.001-10 ug mL' with R2 = 0.999. Recoveries at three different concentration levels (0.1, 1.0 and 5 pg mL"') ranged from 92.0-101.7% with RSD less than of 2.2%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.3 and 1.0 ng mL1, respectively. The developed method was used for the determination of formaldehyde in various flour-based samples.